Identification of salinity responsive genes in lavender by means of cDNA-AFLP
Presently, a world demand exists forlavender as an enormous medicinal plant and provide of essential oils. Freshwater and arable lands are two essential components that inhibit intensive farming of medicinal crops in Iran. Saline water from seas and salty soil may be new sources for agricultural use, notably for medicinal crops.
We sought to extend our information of the Lavandulaangustifolia genome and molecular basis of its salinity tolerance by means of using cDNA amplified fragment dimension polymorphism (cDNA-AFLP) to investigate the changes in plant transcriptomes in response to NaCl.
All acknowledged transcript derived fragments (TDF) have been assigned as novel angustifolia genes related to signal transduction, regulation of gene expression, completely different splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR analysis of the TDFs in response to fully completely different concentrations of NaCl revealed diverse ranges of mRNA of the acknowledged genes on this plant.
Our findings equipped essential insights into the molecular response of angustifolia to salinity.
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Recombinant Virus VEGF E (Orf Virus) Protein, His, E.coli-1mg
Description: A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.
Description: Quantitativesandwich ELISA kit for measuring Human vascular endothelial cell growth factor E, VEGF-E in samples from serum, urine, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human vascular endothelial cell growth factor E, VEGF-E ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Human vascular endothelial cell growth factor E, VEGF-E in samples from serum, urine, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Gentaur's E-Cad ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad. Standards or samples are added to the micro ELISA plate wells and combined with
Gentaur's E-Cad CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad . Standards or samples are added to the micro CLIA plate wells and combined with th
Gentaur's APO-E CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse APO-E . Standards or samples are added to the micro CLIA plate wells and combined with th
Gentaur's E-Cad CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad . Standards or samples are added to the micro CLIA plate wells and combined with th
Gentaur's E-Cad ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat E-Cad. Standards or samples are added to the micro ELISA plate wells and combined with th
Gentaur's E-Cad CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat E-Cad . Standards or samples are added to the micro CLIA plate wells and combined with the
Gentaur's E-Cad ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the micro ELISA plate wells and combined with
Should the Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma or other biological fluids.
Should the Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma or other biological fluids.
Should the Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: This is purified Mouse monoclonal antibody against Hepatitis B virus e antigen for WB, ELISA.
Identification and characterization of a cDNA encoding a gametocyte-specific protein of the avian coccidial parasite Eimerianecatrix
Gametocyte proteins of Eimeria spp. are essential components of the oocyst wall, and some of those proteins have been analysed to ascertain targets of transmission-blocking vaccines in opposition to avian coccidiosis. Throughout the present look at, a cDNA from E. necatrix gametocytes was cloned and sequenced. The cDNA is 1,473 bp in dimension and encodes a 490-amino-acid protein containing a tyrosine-serine (Tyr/Ser)-rich space and a proline-methionine (Skilled/Met)-rich space.
A quantitative real-time PCR (qPCR) analysis confirmed that the cDNA is expressed solely all through gametogenesis. A fraction containing the Tyr/Ser-rich space (rEnGAM59) was expressed in Escherichia coli BL21 (DE3) cells. Immunoblotting confirmed that rEnGAM59 was acknowledged by the serum of convalescent chickens after an an infection with E. necatrix, and that an anti-rEnGAM59 antibody acknowledged a ∼59 kDa protein and
two completely different proteins (∼35 kDa and ∼33 kDa) in gametocyte extracts. An immunofluorescence assay confirmed that the anti-rEnGAM59 antibody acknowledged wall-forming our our bodies throughout the macrogametocytes and oocyst partitions. An in vivo vaccination and drawback trial was carried out to test the potential utility of rEnGAM59 as a vaccine.
Immunized chickens carried out larger than the unimmunized and challenged (constructive administration) chickens. The intestinal lesion scores have been significantly lower throughout the immunized groups than throughout the constructive administration group (P < 0.05). In distinction, the physique weight optimistic points (BWG) have been significantly bigger throughout the immunized groups than throughout the constructive administration group (P < 0.05). There have been no very important variations throughout the lesion scores and BWG between the groups immunized with rEnGAM59 protein or with keep oocysts (P > 0.05). Chickens immunized with rEnGAM59 protein had a significantly bigger antigen-specific serum IgY response (P < 0.05). rEnGAM59 protein might be utilized as candidate antigen to develop a recombinant coccidiosis vaccine.
Reverse genetics of rotaviruses: Era of recombinant human rotaviruses from simply 11 cDNAs encoding the rotavirus genome
A totally plasmid-based reverse genetics system for animal rotavirus was established very recently. We improved the reverse genetics system to generate recombinant rotavirus by transfecting solely 11 T7 plasmids for its 11 genes beneath the scenario of accelerating the ratio (3- or 5-fold) of the cDNA plasmids for NSP2 and NSP5 genes (11-plasmid system).
Utilizing this extraordinarily surroundings pleasant system, we engineered the first infectious recombinant rotaviruses harboring fluorescent (EGFP and mCherry) protein genes. Together with these recombinant animal viruses, the first infectious recombinant human rotavirus (stress KU (G1P[8])) was moreover generated with the 11-plasmid system with some modifications.
The availability of recombinant human rotaviruses will current a genetic platform for a higher understanding of the replication, pathogenicity, and completely different natural traits of this medically very important virus and permit the rational enchancment of next-generation human rotavirus vaccines.
Should the Human Complement Factor B (CFB) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Should the Human Complement Factor B (CFB) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Should the Mouse Complement Factor B (CFB) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Should the Mouse Complement Factor B (CFB) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Should the Rat Complement Factor B (CFB) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Should the Rat Complement Factor B (CFB) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CFB (Center). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody against CFB. Recognizes CFB from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against Cfb. Recognizes Cfb from Mouse, Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against CFB. Recognizes CFB from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human CFB / Complement Factor B . This antibody is tested and proven to work in the following applications:
Description: This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.
Description: This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.
Complement Factor B (CFB) Polyclonal Antibody (Human)
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against Cfb. Recognizes Cfb from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against Cfb. Recognizes Cfb from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against CFB. Recognizes CFB from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against CFB. Recognizes CFB from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against Cleaved-CFB (K260). Recognizes Cleaved-CFB (K260) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000
Complement Factor B (CFB) Polyclonal Antibody (Human), APC
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