Identification of cell inhibitors in opposition to Chikungunya virus replication by a cDNA expression cloning combined with MinION sequencing
cDNA expression cloning has been confirmed to be a powerful technique throughout the look for cell parts that administration virus replication. On this look at, cDNA library screening using a pool of cDNA derived from interferon-treated human cells was combined with the MinION sequencer to determine cell genes inhibiting Chikungunya virus (CHIKV) replication.
Downside an an infection of CHIKV to Vero cells transduced with the cDNA library produced virus-resistant cells. Then, the MinION sequence of cDNAs extracted from the surviving cells revealed that the open finding out frames of TOM7, S100A16, N-terminally truncated kind of ECI1 (ECI1ΔN59), and RPL29 have been inserted in a lot of the cells.
Importantly, the transient expression of TOM7, S100A16, and ECI1ΔN59 was found to inhibit the replication of CHIKV in Huh7 cells, indicating that these cell parts have been doubtlessly anti-CHIKV molecules.
Thus, our look at demonstrated that cDNA expression cloning combined with the MinION sequencer allowed a quick and full detection of cell inhibitors in opposition to CHIKV.
Description: A polyclonal antibody against XPO6. Recognizes XPO6 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
Description: A polyclonal antibody against XPO6. Recognizes XPO6 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC
Description: The protein encoded by this gene is a member of the importin-beta family. Members of this family are regulated by the GTPase Ran to mediate transport of cargo across the nuclear envelope. This protein has been shown to mediate nuclear export of profilin-actin complexes. A pseudogene of this gene is located on the long arm of chromosome 14. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Corn
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Orange
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Potato
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Rice
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Identification of a novel KIR3DL3*064 allele by cDNA cloning and sequencing
Aim: To report on a novel KIR3DL3 allele acknowledged in a southern Han Chinese language language specific individual.
Methods: Peripheral blood sample was collected from a voluntary blood donor with inconclusive final result by KIR3DL3 sequence-based typing (SBT). Complete mRNA was extracted and subjected to reverse transcription to accumulate KIR3DL3 cDNA, which was then amplified by PCR with a pair of KIR3DL3-specific primers. The product was subjected to cDNA cloning and sequencing.
Outcomes: cDNA cloning and sequencing have acknowledged a wide-type KIR3DL3*00802 allele and a novel KIR3DL3*064 allele. The latter differed from KIR3DL3*00601 by a missense variant at codon 374[c.1184 C>T (p.Thr374Ile)] in exon 9. The novel KIR3DL3 allele has been formally assigned by the KIR subcommittee of World Properly being Group Nomenclature Committee for parts of HLA system.
Conclusion: cDNA cloning and sequencing is also used to inform aside inconclusive ends in KIR3DL3 SBT with a function to determine novel KIR alleles.
Apple Russet Ring and Apple Inexperienced Crinkle Diseases: Achievement of Koch’s Postulates by Virome Analysis, Amplification of Full-Measurement cDNA of Viral Genomes, in vitro Transcription of Infectious Viral RNAs, and Duplicate of Indicators on Fruits of Apple Bushes Inoculated With Viral RNAs
Apple russet ring and apple inexperienced crinkle are graft-transmitted sicknesses first reported better than 60 years previously, nonetheless at present, no affiliation between a specific virus (variant) and the sickness has been clearly demonstrated.
On this look at, we carried out the following assortment of experiments to determine the causal viruses (variants) of these apple sicknesses; (1) full analysis by next-generation sequencing of all viruses in each apple tree affected with russet ring or inexperienced crinkle sickness,
(2) amplification of full-length genomic cDNA of viruses using primers containing the T3 promoter and the in vitro transcription of infectious viral RNAs, (3) inoculation of viral RNA transcripts to every herbaceous and apple crops, (4) analysis of sequence variants of viruses present in contaminated crops, (5) back-inoculation of sequence variants of candidate viruses to apple seedlings combined with the virus-induced flowering know-how using the apple latent spherical virus vector to breed the symptom on the fruit as shortly as doable, and
(6) reproduction of indicators on the fruits of apple timber inoculated with sequence variants and the re-isolation of each virus variant from apples exhibiting fruit indicators.
The outcomes confirmed that one in all many sequence variants of the apple chlorotic leaf spot virus causes a attribute ring-shaped rust on the fruits of contaminated apple timber and {{that a}} sequence variant of the apple stem pitting virus possibly causes inexperienced crinkle indicators on an contaminated apple fruit.
Thus, we have now been able to fulfill Koch’s postulates to indicate the viral etiology of every the apple russet ring and inexperienced crinkle sicknesses. We moreover recommend an experimental system that will present whether or not or not a virus current in diseased tissues is the pathogen responsible for the sicknesses when the etiology is undetermined.
Human Phospholipid Transfer Protein (PLTP) ELISA Kit
Description: A Monoclonal antibody against Human PLTP (monoclonal) (M01). The antibodies are raised in mouse and are from clone 2F3-G4. This antibody is applicable in WB and IHC
Description: A polyclonal antibody against PLTP. Recognizes PLTP from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:1000-1:2000, WB:1:200-1:1000
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PLTP. Recognizes PLTP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PLTP . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PLTP . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PLTP . This antibody is tested and proven to work in the following applications:
Immunogen information: Synthesized peptide derived from part region of human PLTP protein at amino acid sequence of 180-260
Applications tips:
Description: A polyclonal antibody for detection of PLTP from Human, Mouse. This PLTP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PLTP protein at amino acid sequence of 180-260
Immunogen information: Synthesized peptide derived from part region of human PLTP protein at amino acid sequence of 180-260
Applications tips:
Description: A polyclonal antibody for detection of PLTP from Human, Mouse. This PLTP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PLTP protein at amino acid sequence of 180-260
Immunogen information: Synthesized peptide derived from part region of human PLTP protein at amino acid sequence of 180-260
Applications tips:
Description: A polyclonal antibody for detection of PLTP from Human, Mouse. This PLTP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PLTP protein at amino acid sequence of 180-260
Description: The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Phospholipid Transfer Protein (PLTP) in serum, plasma, tissue homogenates and other biological fluids.
Human Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Phospholipid Transfer Protein (PLTP) in serum, plasma, tissue homogenates and other biological fluids.
Human Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Phospholipid Transfer Protein (PLTP) in serum, plasma, tissue homogenates and other biological fluids.
Human Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Phospholipid Transfer Protein (PLTP) in serum, plasma, tissue homogenates and other biological fluids.
Human Phospholipid Transfer Protein (PLTP) ELISA Kit
Known also as Phospholipid Transfer Protein elisa. Alternative names of the recognized antigen: Lipid transfer protein II
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Phospholipid Transfer Protein (PLTP) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Phospholipid Transfer Protein (PLTP) in serum, plasma and other biological fluids.
Mouse Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Phospholipid Transfer Protein (PLTP) in serum, plasma and other biological fluids.
Mouse Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Phospholipid Transfer Protein (PLTP) in serum, plasma and other biological fluids.
Mouse Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Phospholipid Transfer Protein (PLTP) in serum, plasma and other biological fluids.
Mouse Phospholipid Transfer Protein (PLTP) ELISA Kit
Known also as Phospholipid Transfer Protein elisa. Alternative names of the recognized antigen: Lipid transfer protein II
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Phospholipid Transfer Protein (PLTP) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Rat Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Phospholipid Transfer Protein (PLTP) in serum, plasma, tissue homogenates and other biological fluids.
Rat Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Phospholipid Transfer Protein (PLTP) in serum, plasma, tissue homogenates and other biological fluids.
Rat Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Phospholipid Transfer Protein (PLTP) in serum, plasma, tissue homogenates and other biological fluids.
Rat Phospholipid Transfer Protein (PLTP) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Phospholipid Transfer Protein (PLTP) in serum, plasma, tissue homogenates and other biological fluids.
Rat Phospholipid Transfer Protein (PLTP) ELISA Kit
Known also as Phospholipid Transfer Protein elisa. Alternative names of the recognized antigen: Lipid transfer protein II
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Phospholipid Transfer Protein (PLTP) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PLTP. Recognizes PLTP from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PLTP. Recognizes PLTP from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PLTP. Recognizes PLTP from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Corn
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Orange
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Potato
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Rice
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PLTP (aa470-490). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PLTP (C-term). This antibody is tested and proven to work in the following applications:
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse PLTP in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.